Catechol 2,3-dioxygenase and other meta-cleavage catabolic pathway genes in the “anaerobic” termite gut spirochete Treponema primitia

Microorganisms have evolved a spectacular diversity of metabolisms, some of which allow them to overcome environmental constraints, utilize abundant but inaccessible resources, and drive nutrient cycling in various ecosystems. The termite hindgut microbial community is optimized to metabolize wood and in recent years, the in situ physiological and ecological functions of community members have been researched. Spirochetes are abundant in the termite gut and herein, putative aromatic meta-cleavage pathway genes typical of aerobic pseudomonads were located in genomes of homoacetogenic termite hindgut “anaerobes,” Treponema primitia str. ZAS-1 and ZAS-2. Phylogenetic analyses suggest the T. primitia catechol 2,3-dioxygenase and several other essential meta-pathway genes were acquired from an α-proteobacterium in the distant past to augment several genes T. primitia acquired from anaerobic firmicutes that do not directly catabolize aromatics but can contribute to the final pathway steps. Further, transcripts for each meta-pathway gene were expressed in strictly anaerobic cultures of T. primitia str. ZAS-2 indicative of constitutive pathway expression. Also, the addition of catechol + O_2 to T. primitia liquid cultures resulted in the transient accumulation of trace amounts of the yellow ring cleavage product, hydroxymuconic semialdehyde. This is the first evidence of aromatic ring cleavage in the phylum (division) Spirochetes. Results also support a possible role for T. primitia in termite hindgut O_2/lignin aromatic monomer metabolism. Potential O_2-dependent yet non-respiratory microbial metabolisms have heretofore been overlooked and warrant further investigation. These metabolisms could describe the degradation of plant-derived and other aromatics in microoxic environments, and contribute significantly to carbon turnover

A Robust Waveguide Millimeter-Wave Noise Source

This paper presents the design, fabrication, and characterization of a millimeter-wave noise source for the 160- 210 GHz frequency range. The noise source has been implemented in an E-split-block waveguide package and the internal circuitry was developed on a quartz substrate. The measured excess noise ratio at 200 GHz is 9.6 dB

Localizing transcripts to single cells suggests an important role of uncultured deltaproteobacteria in the termite gut hydrogen economy

Identifying microbes responsible for particular environmental functions is challenging, given that most environments contain an uncultivated microbial diversity. Here we combined approaches to identify bacteria expressing genes relevant to catabolite flow and to locate these genes within their environment, in this case the gut of a “lower,” wood-feeding termite. First, environmental transcriptomics revealed that 2 of the 23 formate dehydrogenase (FDH) genes known in the system accounted for slightly more than one-half of environmental transcripts. FDH is an essential enzyme of H_2 metabolism that is ultimately important for the assimilation of lignocellulose-derived energy by the insect. Second, single-cell PCR analysis revealed that two different bacterial types expressed these two transcripts. The most commonly transcribed FDH in situ is encoded by a previously unappreciated deltaproteobacterium, whereas the other FDH is spirochetal. Third, PCR analysis of fractionated gut contents demonstrated that these bacteria reside in different spatial niches; the spirochete is free-swimming, whereas the deltaproteobacterium associates with particulates. Fourth, the deltaproteobacteria expressing FDH were localized to protozoa via hybridization chain reaction-FISH, an approach for multiplexed, spatial mapping of mRNA and rRNA targets. These results underscore the importance of making direct vs. inference-based gene– species associations, and have implications in higher termites, the most successful termite lineage, in which protozoa have been lost from the gut community. Contrary to expectations, in higher termites, FDH genes related to those from the protozoan symbiont dominate, whereas most others were absent, suggesting that a successful gene variant can persist and flourish after a gut perturbation alters a major environmental niche

Real-Time Detection and Filtering of Radio Frequency Interference On-board a Spaceborne Microwave Radiometer: The CubeRRT Mission

The Cubesat Radiometer Radio frequency interference Technology validation mission (CubeRRT) was developed to demonstrate real-time on-board detection and filtering of radio frequency interference (RFI) for wide bandwidth microwave radiometers. CubeRRT’s key technology is its radiometer digital backend (RDB) that is capable of measuring an instantaneous bandwidth of 1 GHz and of filtering the input signal into an estimated total power with and without RFI contributions. CubeRRT’s on-board RFI processing capability dramatically reduces the volume of data that must be downlinked to the ground and eliminates the need for ground-based RFI processing. RFI detection is performed by resolving the input bandwidth into 128 frequency sub-channels, with the kurtosis of each sub-channel and the variations in power across frequency used to detect non-thermal contributions. RFI filtering is performed by removing corrupted frequency sub-channels prior to the computation of the total channel power. The 1 GHz bandwidth input signals processed by the RDB are obtained from the payload’s antenna (ANT) and radiometer front end (RFE) subsystems that are capable of tuning across RF center frequencies from 6 to 40 GHz. The CubeRRT payload was installed into a 6U spacecraft bus provided by Blue Canyon Technologies that provides spacecraft power, communications, data management, and navigation functions. The design, development, integration and test, and on-orbit operations of CubeRRT are described in this paper. The spacecraft was delivered on March 22nd, 2018 for launch to the International Space Station (ISS) on May 21st, 2018. Since its deployment from the ISS on July 13th, 2018, the CubeRRT RDB has completed more than 5000 hours of operation successfully, validating its robustness as an RFI processor. Although CubeRRT’s RFE subsystem ceased operating on September 8th, 2018, causing the RDB input thereafter to consist only of internally generated noise, CubeRRT’s key RDB technology continues to operate without issue and has demonstrated its capabilities as a valuable subsystem for future radiometry missions

SMAP L-Band Microwave Radiometer: Instrument Design and First Year on Orbit

The Soil Moisture Active Passive (SMAP) L-band microwave radiometer is a conical scanning instrument designed to measure soil moisture with 4 percent volumetric accuracy at 40-kilometer spatial resolution. SMAP is NASA's first Earth Systematic Mission developed in response to its first Earth science decadal survey. Here, the design is reviewed and the results of its first year on orbit are presented. Unique features of radiometer include a large 6-meter rotating reflector, fully polarimetric radiometer receiver with internal calibration, and radio-frequency interference detection and filtering hardware. The radiometer electronics are thermally controlled to achieve good radiometric stability. Analyses of on-orbit results indicate the electrical and thermal characteristics of the electronics and internal calibration sources are very stable and promote excellent gain stability. Radiometer NEdT (Noise Equivalent differential Temperature) less than 1 degree Kelvin for 17-millisecond samples. The gain spectrum exhibits low noise at frequencies greater than 1 megahertz and 1 divided by f (pink) noise rising at longer time scales fully captured by the internal calibration scheme. Results from sky observations and global swath imagery of all four Stokes antenna temperatures indicate the instrument is operating as expected

catechol 2,3-dioxygenase C-terminal domain with extra-domain region ARB output.nex

catechol 2,3-dioxygenase C-terminal domain with extra-domain region ARB output.ne